Reduced Solvent Accessibility and Increased Stability of D-Lac Mutant: A SASA Analysis

Solvent accessible surface area (SASA) analysis revealed that the D-Lac mutant exhibited a lower reduction in SASA compared to the wild-type (WT). This finding implies that the D-Lac mutant has less surface exposure, possibly due to its more compact structure. This compactness could contribute to the enhanced stability observed in the D-Lac mutant. The analysis suggests that the mutations in D-Lac lead to a more tightly packed conformation, shielding it from the solvent environment and potentially increasing its resistance to unfolding or degradation.