Regulation of ARAP1 Transcript Expression in HepG2 Cells via shRNA and Lentiviral Overexpression Systems

This study focuses on modulating the expression levels of different ARAP1 transcripts in HepG2 cells. We utilized shRNA and a lentivirus overexpression system to achieve targeted regulation of specific ARAP1 transcripts. Through these approaches, we successfully generated stable transfected HepG2 cell lines with altered ARAP1 transcript expression. Our findings provide a valuable resource for investigating the distinct roles of ARAP1 isoforms in the context of liver cancer.