Currently, there have been hundreds of studies on alginate lyases, but studies on G-specific alginate lyases are relatively few. Less than 10 endonucleases have been identified, and the degradation mechanisms of some enzymes are unclear, which makes it difficult to meet the production needs in various situations. Considering that there are five G-specific alginate lyases in the PL7 family, constructing an evolutionary tree for all characterized enzymes in the PL7 family reveals that most G-specific enzymes cluster in a branch (the PL7_3 subfamily), providing a possible direction for the discovery of G-specific enzymes

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