用更为地道、科学的英语表达如下内容:Under the dynamic flow media in the microfluidic chip the triple-cell-type endometrial culture self-assembled into a highly interconnected network surrounding organoids IF staining v
Under dynamic flow conditions within a microfluidic chip, a triple-cell-type endometrial culture underwent self-assembly to form a highly interconnected network encompassing organoids. Immunofluorescence staining confirmed the presence of von Willebrand factor (vWF) and CD31 positive endothelial cells, Vimentin positive endometrial stromal cells, and EPCAM positive endometrial epithelial cells within vasculized endometrial organoids (Figure 2E). Proliferative cells were predominantly observed in endometrial epithelial cells (Figure 2E and G). Calcein/PI staining demonstrated that the majority of cells were viable, with a minor population exhibiting slight PI fluorescence, indicative of cellular death (Figure 2F). Immunohistochemistry results further confirmed the existence of micro-vessels surrounding the endometrial organoids (Figure 2G)
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