A 10% leaf homogenate was prepared utilizing physiological saline, and the resulting supernatant was subjected to centrifugation to measure antioxidase. The activities of each enzyme were assessed employing assay kits, in accordance with the guidelines provided by the manufacturer (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Leaf Homogenate Preparation and Antioxidant Enzyme Activity Measurement

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