翻译并用流畅重写： Although flow cytometry is not as popular in spermatology as in other areas of biology and medicine is increasingly being used in this field 1 2 Spermatozoa similarly to blood cells are

Although flow cytometry is not as widely used in spermatology as in other fields of biology and medicine, it is increasingly being employed in this area. Spermatozoa, like blood cells, are individual cells suspended in a liquid (seminal plasma) and are therefore well-suited for flow cytometry analysis. While flow cytometry has been used in spermatology for some time, most applications have been simple combinations of one or two probes and two colors. However, the complexity of flow cytometry analysis in spermatology is increasing, as demonstrated by Umair et al. (2019), who used a multiparametric approach to study the mechanisms of sperm aging during conservation. Their study showed the power of flow cytometry in revealing changes in Ca2+ concentration and plasma membrane fluidity in viable acrosome-intact spermatozoa. This underscores the utility of flow cytometry in studying the biology of spermatozoa and its potential to inform clinical settings. Recent papers also demonstrate the usefulness of flow cytometry in clinical sperm analysis and the study of sperm biology