帮我改写润色文章尽量用高级写作词汇。The energy difference between the improved structure and the single point mutant was calculated using the ligand-free D-Lac structure as the input file Positions with multiple mutati

The dissimilarity in energy levels between the refined configuration and the solitary point mutant was computed by utilizing the ligand-free D-Lac structure as the initial file. Sites with numerous mutations in ΔΔG≤0 R.e.u. were categorized as subpar, and pSUFER was made accessible at (https://psufer.weizmann.ac.il). A selection of residues in close proximity to the binding pocket that were consistently identified as suboptimal were deemed potential targets for evolutionary enhancement in order to augment the stability of the fold