帮我改写润色文章尽量用高级写作词汇。Mutant F308G was used as a template for FuncLib calculations httpfunclibweizmannacil13 The FuncLib design was applied to residue numbers A349 in the D-Lac surrounding of D-PL Essent

The FuncLib calculations were conducted using Mutant F308G as a template (http://funclib.weizmann.ac.il/), with a focus on the residue numbers surrounding A349 in the D-Lac of D-PL. Essential amino acid residue D311 was also taken into consideration. Multiple sequence alignments were executed with default parameters, and a minimum of 2 and a maximum of 5 mutations were permitted for each design. The FuncLib algorithm ultimately produced 1000 mutant combinations, from which the top 50 structures were selected based on their scores