帮我润色尽量用高级词汇。The enzyme specific activity was defined and determined as previously described 1 An appropriate amount of pure enzyme was added to 14 mL of a 10 wv DL-PL substrate prepared in 1 M Tris-HC

The enzyme's specific activity was defined and determined in accordance with previously established protocols [1]. A suitable quantity of pure enzyme was introduced into 14 mL of a 10% (w/v) DL-PL substrate, which had been prepared in 1 M Tris-HCl buffer with a pH of 7.5. The reaction was subsequently terminated by heating the mixture to 100°C for a duration of 3 minutes, followed by filtration and measurement via HPLC. One unit of D-Lacs activity was defined as the quantity of enzyme required to liberate 1 μmol of D-PA per minute. Samples were assessed in a single layer using a Waters HPLC system that was fitted with a Chiralcel IG-3 column (4.6 mm × 250 mm, 3 µm, Diacel, Tokyo, Japan). The eluent employed consisted of formic acid/methanol (0.1%, 70:30, v/v), and was administered at a flow rate of 0.3 mL/min at a temperature of 35°C, while detection was performed at a wavelength of 210 nm