帮我润色尽量使用高级写作词汇。Single colonies from LBkan agar plates were picked with sterile toothpicks and cultured in cell culture tubes containing LBkan overnight at 37°C at 220 rpm An appropriate amount of over

Single colonies were carefully selected from LBkan agar plates using sterile toothpicks and subsequently cultured in cell culture tubes containing LBkan medium overnight at 37°C with agitation at 220 rpm. Following this, an appropriate volume of the overnight culture was transferred to fresh LBkan medium and subjected to shaking at 37°C and 220 rpm for a period of 2.5-3 hours. Once the OD600 reached a value of approximately 0.6-0.8, the culture was induced with 0.4mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and incubated at 20°C for a duration of 18 hours