I want you to act as an academic journal editor Please rephrase the paragraph from an academic angle based on the writting style of the Nature journalTotal proteins were separated by electrophoresis o

The separation of total proteins was conducted via electrophoresis on SDS-polyacrylamide gels with a range of 8 to 12%. Nitrocellulose membranes were subsequently utilized for the transfer of these proteins. Following blocking with 5% nonfat milk, primary antibody probing was performed overnight at 4 °C, with a subsequent 1-hour incubation with secondary antibody at 37 °C. Visualization of samples was carried out through the use of pierce™ECL Western Blotting Substrate from ThermoFisher (UT, USA) and an Image analysis system from Azure Biosystems (USA)