I want you to act as an academic journal editor Please rephrase the paragraph from an academic angle based on the writting style of the Nature journalTo assess the toxicity of PSNPs PI staining was us

The present study aimed to evaluate the toxicity of PSNPs through the application of PI staining methodology to identify cells that exhibited compromised cell membranes. Following exposure to PSNPs, cellular samples were collected through trypsinization and washed with PBS twice. The cellular spheres were subsequently suspended in DMEM medium that contained a 3 μM PI staining solution. After a 15-minute incubation period at room temperature, the stained cells underwent flow cytometry analysis to enable further characterization