what solvent system normal phase solvent or reverse phase solvent is used to separate the Curvularin-7-O-α-D-glucopyranoside and Dehydrocurvularin by Sephadex LH-20 column

Sephadex LH-20 is a type of gel filtration chromatography column that is typically used for the separation of small molecules based on their molecular weight. It is a type of normal phase chromatography, which means that it uses a non-polar solvent system to separate the molecules based on their polarity.

In this case, since Curvularin-7-O-α-D-glucopyranoside and Dehydrocurvularin are both small molecules, they can be separated using a Sephadex LH-20 column. The most common solvent system used for this type of column is a mixture of polar and non-polar solvents, such as methanol and water. This is because the Sephadex LH-20 column has a hydrophobic matrix that interacts with the non-polar components of the sample, while the polar components are eluted more quickly.

Therefore, a normal phase solvent system would be used to separate Curvularin-7-O-α-D-glucopyranoside and Dehydrocurvularin by Sephadex LH-20 column