what solvent system normal phase solvent or reverse phase solvent is used to separate the Curvularin-7-O-α-D-glucopyranoside and Dehydrocurvularin by Sephadex LH-20 column and what the order of compou

Sephadex LH-20 column is a type of gel filtration column that is typically used in normal phase chromatography. Therefore, the solvent system used to separate Curvularin-7-O-α-D-glucopyranoside and Dehydrocurvularin is a normal phase solvent system, which typically consists of non-polar to polar solvents such as hexane, ethyl acetate, and methanol.

The order in which the compounds come out from the column depends on their molecular weight and polarity. Generally, larger and more polar compounds will elute first, followed by smaller and less polar compounds. Therefore, Curvularin-7-O-α-D-glucopyranoside, which has a larger molecular weight and is more polar than Dehydrocurvularin, is expected to elute first from the column, followed by Dehydrocurvularin