However subjecting the multiple combinatorial mutants obtained from FuncLib results to gene synthesis or Gibbs assembly makes FuncLib inefficient and uneconomical in the process and may lead to unfavo

Nevertheless, the application of FuncLib in generating multiple combinatorial mutants may prove to be inefficient and uneconomical when subjected to gene synthesis or Gibbs assembly. This is due to the potential adverse effects of upregulation resulting from the mutation, which may further impede the efficacy of FuncLib.