In summary a total of 45 mutants were obtained by evolution using the CSFp strategy of which 69 had D-Lac activity and 16 mutants had more than an order of magnitude increase in activity of which the

To summarize, the CSFp approach yielded a total of 45 mutants through evolutionary processes, with a notable 69% exhibiting D-Lac activity. Among these, 16 mutants displayed activity levels exceeding an order of magnitude, with the top-performing variant, N, demonstrating a remarkable 56-fold increase in activity.