Soil N mineralization rates are regulated by several factors such as temperature pH and microbial biomass Elrys et al 2021 The effect of microbial load has not been described in detail in earlier stud

Soil nitrogen (N) mineralization rates are influenced by multiple factors, including temperature, pH, and microbial biomass (Elrys et al., 2021). However, the impact of microbial load on large-scale soil N mineralization has not been thoroughly explored in previous studies (Colman and Schimel, 2013; Liu et al., 2017). Accounting for microbial load in N mineralization analysis is crucial, as it is an essential factor in regulating Nmin (Schimel and Bennett, 2004). Incorporating microbial load in predictions can enhance the accuracy of Nmin predictions by up to 19% (Li et al., 2019). However, the role of soil structure in Nmin remains poorly understood. Modeling the intricate microbial and environmental mechanisms governing Nmin is challenging, especially on a global scale. An in-depth comprehension of the geographic patterns and regulatory mechanisms of Nmin worldwide is crucial for better understanding the global spatial variability of N mineralization