The reaction was allowed to proceed at 30 °C for 20 min after which the enzyme was inactivated by boiling The samples were detected in a single layer using a Waters HPLC system equipped with a Chiralc

The reaction was permitted to proceed at a temperature of 30 °C for a duration of 20 minutes, following which the enzyme was deactivated through boiling. The samples were analyzed in a singular layer utilizing a Waters HPLC system, which was equipped with a Chiralcel IG-3 column (4.6 mm × 250 mm, 3 µm, Diacel, Tokyo, Japan). The eluent used was a mixture of formic acid and methanol (0.1%, 70:30, v/v), and the flow rate was set to 0.3 mL/min at a temperature of 35 °C, with a wavelength of 210 nm being employed for detection purposes