pcr反应采用了25微升的反应体系该体系包括上下游引物各1微升3微升的BSA1微升的DNA模版125微升的TAQPLUS及65微升的ddhao。翻译成英文
The PCR reaction used a 25 μL reaction system, which included 1 μL of upstream and downstream primers each, 3 μL of BSA, 1 μL of DNA template, 12.5 μL of TAQPLUS, and 6.5 μL of ddH2O.
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