Microemulsion droplets were generated in a microfluidic PDMS device with flow-focus channel geometry In order to avoid cross contamination during droplet formation new devices were used when droplet

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The microfluidic chip was connected to an inverted fluorescence microscope (Zeiss Observer Z1) equipped with a temperature-controlled stage (Okolab). Images were acquired using a CCD camera (Hamamatsu). The fluorescence signal from the bacteria was excited with a 488 nm laser and emitted light was filtered with a 525/50 nm bandpass filter. Images were acquired every 5 minutes for a total of 24 hours. Data analysis was performed using custom Matlab scripts. Droplets were tracked over time and the fluorescence intensity of each droplet was measured. The data was then used to generate plots of the growth dynamics of the bacteria in the different droplets.