Please help me to polish this technical paper to make it read more smooth and reasonable The endogenous peroxynitrite imaging was evaluated in HepG2 cells As shown in Figure 5c after incubation with

The study evaluated the effectiveness of endogenous peroxynitrite imaging in HepG2 cells. The results, shown in Figure 5c, demonstrated that HepG2 cells exhibited strong yellow fluorescence at 514 nm after incubation with SiQDs-(K/L-P)ox for 30 min. Furthermore, when the cells were stimulated to produce ONOO- by adding 10 μg/mL of LPS, the yellow fluorescence intensity decreased and the red fluorescence was almost completely quenched after 2 h.

To ensure the accuracy of the results, control tests were conducted by adding DMSO (300 μM) to prevent endogenous ONOO- production before incubating the cells with LPS. The yellow fluorescence intensity of 514 nm channel did not change after DMSO and LPS were incubated for 1 h and 2 h, indicating that the observed changes in fluorescence were due to the production of ONOO-.

The processed data in the Spec3 channel visually demonstrated the changes in fluorescence intensity of the SiQDs-(K/L-P)ox in the HepG2 cells. These findings suggest that the developed SiQDs-(K/L-P)ox probe can be utilized to monitor the changes in endogenous ONOO- levels in living cancer cells in real-time.

Overall, these experiments provide valuable insights into the effectiveness of endogenous peroxynitrite imaging in HepG2 cells, and suggest that the SiQDs-(K/L-P)ox probe holds promise as a powerful tool for monitoring changes in endogenous ONOO- levels in living cancer cells.