Please help me to polish this technical paper to make it read more smooth and reasonable The standard curves for the detection of ONOO- using the SiQDs-KL-Pox was showed in Figure 4d With increasing
The standard curves for the detection of ONOO- using SiQDs-(K/L-P)ox were presented in Figure 4d. It was observed that as the concentration of ONOO- increased, the fluorescence intensity at 480 nm gradually decreased. The insert diagram in Figure 4e further demonstrated that the emission light of SiQDs-(K/L-P)ox darkened with increasing ONOO- concentration under UV irradiation. The line diagram in Figure 4e indicated that the concentration range of ONOO- between 100 – 300 μM showed low ∆I, which could lead to inaccurate measurements. Therefore, concentrations greater than 100 μM were not used as the standard curve.
Figure 4f and g demonstrated that the probe established two linear relationships between the concentration range of 2 – 100 μM. The first linear relationship was observed between 2 – 10 μM, with an R2 value of 0.996 and a limit of detection (LOD) of 99 nM. The second linear relationship was observed between 20 – 100 μM, with an R2 value of 0.994 (based on a signal-to-noise ratio S/N = 3).
To test the specificity of SiQDs-(K/L-P)ox, interference experiments were conducted and presented in Figure 4h. The probe solution was incubated with common metal ions, amino acids and reactive oxygen species for 10 minutes at 30 °C. The mixture was then irradiated with a 380 nm laser to determine the PL intensity of the various substances. The results of the interference experiments showed that all substances, except ONOO-, had a negligible effect on the detection of ONOO- and the probe had a good fluorescence response.
Overall, the SiQDs-(K/L-P)ox probe exhibited good sensitivity and specificity in detecting ONOO-. The established standard curves and linear relationships between the concentration range of 2 – 100 μM make this probe a promising tool for detecting ONOO- in biological and environmental samples.
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