用SCI期刊标准翻译：烟草疫霉菌Phytophthora parasitica属卵菌科疫霉属由烟草疫霉菌引起的烟草黑胫病是烟草重要病害之一对烟草毁灭性极强其分布范围广泛对烟草种植和生产造成巨大的损失选育黑胫病高抗品种是最为经济安全有效的一种烟草黑胫病防控措施手段。因此筛选鉴定有效的烟草黑胫病抗性基因通过基因工程办法选育烟草黑胫病高抗品种具有重要意义。TCP转录因子是植物特有的一类转录因子家族其在植

Phytophthora parasitica is an oomycete fungus belonging to the family Phytophthora, and tobacco black shank disease caused by P. parasitica is one of the most important diseases of tobacco, which is highly destructive and widely distributed, causing significant losses in tobacco cultivation and production. Breeding tobacco varieties with high resistance to black shank disease is the most economically safe and effective measure for its control. Therefore, identifying and selecting effective resistance genes against tobacco black shank disease, and breeding high-resistant tobacco varieties using genetic engineering methods is of great significance. TCP transcription factors are a unique family of transcription factors specific to plants, which play important roles in plant growth, development, and biotic stress responses. In this study, tobacco plants were inoculated with P. parasitica, and samples were taken at different time points for transcriptome sequencing to screen for differentially expressed genes. Through GO function and KEGG pathway enrichment analysis, the biological processes and signaling pathways related to resistance were explored. Furthermore, candidate NtTCP genes related to black shank disease resistance were screened, and their response patterns to P. parasitica infection were analyzed. The preliminary identification of the effect of candidate NtTCP genes on tobacco resistance to P. parasitica was conducted by transiently expressing them in Nicotiana benthamiana and inoculating them with P. parasitica. In addition, this study constructed transgenic tobacco plants overexpressing NtTCP13 and NtTCP36 in the susceptible cultivar 'Honghuadajinyuan', and further investigated the functions of NtTCP13 and NtTCP36 in tobacco black shank disease resistance using physiological and biochemical methods. This study provides a foundation for further understanding the mechanism of tobacco resistance to black shank disease. The specific results of this study are as follows: