常用的双分子荧光互补质粒包括pEGFP-C1/pEYFP-C1、pECFP-C1/pEYFP-C1、pEGFP-N1/pEYFP-N1等。其中,pEGFP-C1/pEYFP-C1和pECFP-C1/pEYFP-C1是最常用的双分子荧光互补质粒,适用于研究蛋白质相互作用、蛋白质定位等问题。

参考文献:

  1. Clontech. pEGFP-C1 Vector [DB/OL]. https://www.takarabio.com/products/gene-function/reporter-assays/fluorescent-reporters/green-fluorescent-protein-egfp/p-egfp-c1-vector.
  2. Clontech. pEYFP-C1 Vector [DB/OL]. https://www.takarabio.com/products/gene-function/reporter-assays/fluorescent-reporters/yellow-fluorescent-protein-eyfp/p-eyfp-c1-vector.
  3. Clontech. pECFP-C1 Vector [DB/OL]. https://www.takarabio.com/products/gene-function/reporter-assays/fluorescent-reporters/cyan-fluorescent-protein-ecfp/p-ecfp-c1-vector.
  4. Clontech. pEYFP-N1 Vector [DB/OL]. https://www.takarabio.com/products/gene-function/reporter-assays/fluorescent-reporters/yellow-fluorescent-protein-eyfp/p-eyfp-n1-vector.
  5. Clontech. pEGFP-N1 Vector [DB/OL]. https://www.takarabio.com/products/gene-function/reporter-assays/fluorescent-reporters/green-fluorescent-protein-egfp/p-egfp-n1-vector.
双分子荧光互补常用的的质粒有哪些,并给出参考文献?

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