蛋白的亚细胞定位中免疫荧光染色常用质粒是哪些，并给出参考文献？

常用的质粒包括pEGFP-C1、pDsRed2-N1、pCMV-Myc和pFLAG-CMV2等。这些质粒可以用于将荧光蛋白标记在目标蛋白的N-或C-端，从而实现目标蛋白的免疫荧光染色。

参考文献：

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4. Kodama Y, Hu CD. Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives. Biotechniques. 2012;53(5):285-298.