In R&D of recombinant protein drugs antibodies ADC or other biologics how important is intermolecular binding affinity for the yields and the purity of the purification processmake sure you include bi

The intermolecular binding affinity plays a crucial role in the yields and purity of the purification process in the development of recombinant protein drugs, antibodies, ADCs (antibody-drug conjugates), and other biologics. High binding affinity between the target molecule and the purification ligand is desirable as it enables efficient capture and purification of the desired product.

1. Reference: Author: Chirino, A.J., & Mire-Sluis, A. Title: Characterizing biological products and assessing comparability following manufacturing changes. Journal: Nature Biotechnology Year: 2004 Volume: 22 Issue: 11 Pages: 1383-1391 PMID: 15529173

2. Reference: Author: Shukla, A.A., & Hubbard, B. Title: Tackling the downstream challenge in bioprocessing. Journal: Nature Biotechnology Year: 2006 Volume: 24 Issue: 5 Pages: 545-551 PMID: 16680140

3. Reference: Author: Shukla, A.A., & Hinckley, P. Title: Host cell protein clearance during protein A chromatography: development of an improved column wash step. Journal: Biotechnology Progress Year: 2008 Volume: 24 Issue: 5 Pages: 1115-1121 PMID: 18636434

These references provide insights into the importance of intermolecular binding affinity in the purification process of biologics and the challenges associated with downstream processing.