翻译：施用盖氏假单胞菌PG-9后苹果根系内生细菌群落结构分析试验处理同12。于2022年8月15日采用五点取样法随机选取长势基本一致的苹果树进行苹果根系采样距树干50 cm范围内在同一方用铁锨挖至部分根系裸露剪下部分根系抖掉周围土壤每样点重复3次带回实验室消毒后-80℃保存备用。苹果根系内生细菌群落委托上海美吉生物医药技术有限公司进行试验OTU聚类。

Analysis of the native bacterial community structure in apple root system after application of Pseudomonas fluorescens strain PG-9

The experimental procedure was the same as described in section 1.2. On August 15, 2022, a five-point sampling method was used to randomly select apple trees with similar growth conditions for apple root sampling. Within a 50 cm range from the tree trunk, a shovel was used to expose part of the root system in the same direction. Partial root samples were cut and the surrounding soil was shaken off. Each sampling point was repeated three times. The samples were then taken back to the laboratory, disinfected, and stored at -80°C for further use. The analysis of the native bacterial community in the apple root system was entrusted to Shanghai Meiji Biopharmaceutical Technology Co., LTD for OTU clustering