用科技论文语言改写并缩写下面内容：Sulfosalicylic acid was used to extract proline Proline can react with an acidic ninhydrin solution and become red after heating and then once extracted by toluene the absorbance can

Sulfosalicylic acid was utilized as an extraction agent for proline. Subsequently, proline underwent a reaction with an acidic ninhydrin solution, resulting in a red color after heating. The extract was then collected using toluene and measured for absorbance at 520 nm via spectrophotometry. The anthrone colorimetry method was employed to determine the soluble sugar content. The soluble sugar was extracted following the guidelines of the soluble sugar assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and the absorbance was recorded at 625 nm. The corresponding soluble sugar content was calculated based on the glucose content in the standard curve. The protein and Coomassie brilliant blue (G-250) combination can form a blue compound complex under acidic solution conditions, which exhibits a characteristic absorption peak at 595 nm. The protein concentration is directly proportional to the color intensity of the solution, and the protein content can be measured using the standard curve (Runyon et al., 2015). The spectrophotometer and soluble protein assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) were utilized for this operation