To obtain high-purity oligosaccharides from the products of Aly7Ce enzyme hydrolysis of brown algae, a preparative-grade gel column (30 PG) can be used for separation. 1 g of brown algae can produce high-purity ΔG 0.182 g, ΔGG 0.147 g, and ΔGGG 0.013 g oligosaccharides, with purity detected by the HPAEC-PAD/MS method described in section 1.5, where the peak area of the target oligosaccharide accounts for more than 80% of the total peak area.


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