Co-Immunoprecipitation Co-Immunoprecipitation principle and experimental method - EnglishPrincipleCo-Immunoprecipitation Co-IP was developed from the immunoprecipitation technique with which Co-IP sha

ImmunoPrecipitation Assay) bufferl Protease inhibitorsl Antibodies against bait and prey proteinsl Protein A/G agarose beadsExperimental Method:1. Cells expressing the bait and prey proteins are lysed in RIPA buffer with protease inhibitors.2. The lysate is pre-cleared by incubation with protein A/G agarose beads.3. The pre-cleared lysate is incubated with an antibody against the bait protein, which is bound to protein A/G agarose beads.4. The lysate is incubated with the antibody-bead complex and then centrifuged to isolate the immunocomplex.5. The immunocomplex is washed to remove non-specifically bound proteins.6. The immunoprecipitated proteins are eluted from the complex and analyzed by Western blotting or mass spectrometry to identify the prey protein