Buffer Solutions with Polyethylene Glycol and Jeffamine for Crystallization

Buffer Solutions for Protein Crystallization

This document provides a detailed list of buffer solutions commonly used for protein crystallization. Each solution comprises a specific buffer component, pH, and a defined concentration of either Polyethylene Glycol (PEG) or Jeffamine.

A Series

• A2 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 38% v/v Polyethylene glycol 200
• A3 0.1 M HEPES pH 7.5, 42% v/v Polyethylene glycol 200
• A5 0.1 M BIS-TRIS pH 6.5, 25% v/v Polyethylene glycol 300
• A9 0.1 M Tris pH 8.0, 30% v/v Polyethylene glycol 400
• A12 0.1 M BIS-TRIS propane pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 550

B Series

• B9 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 1,500
• B12 0.1 M BIS-TRIS propane pH 9.0, 20% w/v Polyethylene glycol monomethyl ether 5,000

C Series

• C2 0.1 M Imidazole pH 7.0, 20% v/v Jeffamineﾮ ED-2001 pH 7.0
• C3 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamineﾮ ED-2001 pH 7.0
• C9 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000
• C11 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
• C12 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000

D Series

• D2 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000
• D5 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 16% w/v Polyethylene glycol 8,000
• D9 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000
• D11 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000
• D12 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000

E Series

• E4 28% v/v 2-Propanol, 0.1 M BIS-TRIS pH 6.5, 3% v/v Polyethylene glycol 200
• E5 20% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 2% v/v Polyethylene glycol 200
• E6 10% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 400
• E7 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 400
• E8 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 18% v/v Polyethylene glycol 400
• E9 0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400
• E12 0.15 M DL-Malic acid pH 7.0, 0.1 M Imidazole pH 7.0, 22% v/v Polyethylene glycol monomethyl ether 550

F Series

• F1 0.1 M Succinic acid pH 7.0, 0.1 M BICINE pH 8.5, 30% v/v Polyethylene glycol monomethyl ether 550
• F2 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 1,000
• F3 0.1 M Sodium malonate pH 8.0, 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol 1,000
• F5 0.2 M L-Proline, 0.1 M HEPES pH 7.5, 24% w/v Polyethylene glycol 1,500
• F6 10% v/v 2-Propanol, 0.1 M BICINE pH 8.5, 30% w/v Polyethylene glycol 1,500
• F7 0.1 M Sodium chloride, 0.1 M BIS-TRIS propane pH 9.0, 25% w/v Polyethylene glycol 1,500
• F10 0.2 M Ammonium citrate tribasic pH 7.0, 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
• F11 4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000

G Series

• G2 2% v/v Tacsimate pH 7.0, 5% v/v 2-Propanol, 0.1 M Imidazole pH 7.0, 8% w/v Polyethylene glycol 3,350
• G3 2% v/v 1,4-Dioxane, 0.1 M Tris pH 8.0, 15% w/v Polyethylene glycol 3,350
• G4 18% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 4,000
• G5 6% v/v Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% w/v Polyethylene glycol 4,000
• G6 0.2 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.0, 18% w/v Polyethylene glycol monomethyl ether 5,000
• G9 4% v/v 2-Propanol, 0.1 M BIS-TRIS propane pH 9.0, 20% w/v Polyethylene glycol monomethyl ether 5,000

H Series

• H3 10% v/v Polyethylene glycol 200, 0.1 M BIS-TRIS propane pH 9.0, 18% w/v Polyethylene glycol 8,000
• H5 0.4 M Sodium malonate pH 6.0, 0.1 M MES monohydrate pH 6.0, 0.5% w/v Polyethylene glycol 10,000
• H6 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS pH 6.5, 10% w/v Polyethylene glycol 10,000
• H7 5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 10,000
• H8 0.2 M Ammonium acetate, 0.1 M Tris pH 8.0, 16% w/v Polyethylene glycol 10,000
• H12 3% w/v Dextran sulfate sodium salt, 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 20,000

This list serves as a starting point for your crystallization experiments. You can adjust the buffer components, pH, and concentration of PEG or Jeffamine to fine-tune the crystallization conditions for your specific protein of interest.