Buffer Solutions with Polyethylene Glycol and Jeffamineﾮ for Crystallization and Protein Purification

Buffer Solutions for Crystallization and Protein Purification

This list provides a comprehensive range of buffer solutions commonly used for crystallization and protein purification. Each entry includes the buffer components, pH, and concentration of the relevant polyethylene glycol (PEG) or Jeffamineﾮ polymer. Please note that this is not an exhaustive list and specific conditions may require further optimization.

Polyethylene Glycol (PEG) Buffers

• C3: 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamineﾮ ED-2001 pH 7.0
• C9: 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000
• C11: 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
• C12: 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000
• D2: 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000
• D5: 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 16% w/v Polyethylene glycol 8,000
• D9: 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000
• D11: 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000
• D12: 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000

Jeffamineﾮ Buffers

• E4: 28% v/v 2-Propanol, 0.1 M BIS-TRIS pH 6.5, 3% v/v Polyethylene glycol 200
• E5: 20% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 2% v/v Polyethylene glycol 200
• E6: 10% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 400
• E7: 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 400
• E8: 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 18% v/v Polyethylene glycol 400
• E9: 0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400
• E12: 0.15 M DL-Malic acid pH 7.0, 0.1 M Imidazole pH 7.0, 22% v/v Polyethylene glycol monomethyl ether 550

Additional Buffers with Complex Components

• F1: 0.1 M Succinic acid pH 7.0, 0.1 M BICINE pH 8.5, 30% v/v Polyethylene glycol monomethyl ether 550
• F2: 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 1,000
• F3: 0.1 M Sodium malonate pH 8.0, 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol 1,000
• F5: 0.2 M L-Proline, 0.1 M HEPES pH 7.5, 24% w/v Polyethylene glycol 1,500
• F6: 10% v/v 2-Propanol, 0.1 M BICINE pH 8.5, 30% w/v Polyethylene glycol 1,500
• F7: 0.1 M Sodium chloride, 0.1 M BIS-TRIS propane pH 9.0, 25% w/v Polyethylene glycol 1,500
• F10: 0.2 M Ammonium citrate tribasic pH 7.0, 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
• F11: 4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000
• G2: 2% v/v Tacsimate pH 7.0, 5% v/v 2-Propanol, 0.1 M Imidazole pH 7.0, 8% w/v Polyethylene glycol 3,350
• G3: 2% v/v 1,4-Dioxane, 0.1 M Tris pH 8.0, 15% w/v Polyethylene glycol 3,350
• G4: 18% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 4,000
• G5: 6% v/v Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% w/v Polyethylene glycol 4,000
• G6: 0.2 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.0, 18% w/v Polyethylene glycol monomethyl ether 5,000
• G9: 4% v/v 2-Propanol, 0.1 M BIS-TRIS propane pH 9.0, 20% w/v Polyethylene glycol monomethyl ether 5,000
• H3: 10% v/v Polyethylene glycol 200, 0.1 M BIS-TRIS propane pH 9.0, 18% w/v Polyethylene glycol 8,000
• H5: 0.4 M Sodium malonate pH 6.0, 0.1 M MES monohydrate pH 6.0, 0.5% w/v Polyethylene glycol 10,000
• H6: 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS pH 6.5, 10% w/v Polyethylene glycol 10,000
• H7: 5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 10,000
• H8: 0.2 M Ammonium acetate, 0.1 M Tris pH 8.0, 16% w/v Polyethylene glycol 10,000
• H12: 3% w/v Dextran sulfate sodium salt, 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 20,000

This information is provided for general reference only. It is always recommended to conduct thorough research and optimize buffer conditions based on the specific requirements of your experiment and target protein.