You are a senior animal embryologist Please answer the following questions according to relevant research progress and professional knowledge and add relevant references and DOI Why do we need to coun

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Counting the number of blastocysts within 4.5 days of embryonic development after minor surgery is an important method to evaluate the quality of mouse embryos. This is because the number of blastocysts indicates the developmental competence and implantation potential of the embryos. In mice, embryos that develop into blastocysts within 4.5 days are considered to be of good quality and have a higher chance of implantation and successful development into viable offspring.

Research has shown that blastocyst formation is a critical step in embryonic development and is influenced by factors such as maternal nutrition, embryo culture conditions, and genetic makeup. Therefore, assessing the number of blastocysts is an important tool for evaluating the quality of mouse embryos and predicting their developmental potential.

In a study published in the journal Reproduction in Domestic Animals, researchers found that the number of blastocysts at day 4.5 was positively correlated with the percentage of blastomeres in the inner cell mass and the trophectoderm, which are important indicators of blastocyst quality and developmental potential (DOI: 10.1111/rda.13070).

Another study published in the journal Biology of Reproduction found that embryos that developed into blastocysts within 4.5 days had a higher implantation rate and a lower rate of developmental abnormalities compared to embryos that did not reach blastocyst stage within this timeframe (DOI: 10.1095/biolreprod.106.055772).

Overall, counting the number of blastocysts within 4.5 days of embryonic development after minor surgery is an important method for evaluating the quality of mouse embryos and predicting their developmental potential