Transcriptome Analysis Reveals Host Defense Mechanisms Against Klebsiella pneumoniae Infection in Mauremys mutica

In this study, we aimed to confirm the impact of Mauremys mutica infection caused by Klebsiella pneumoniae. We identified several differentially expressed genes (DEGs) related to immunity, antioxidant activity, and carbon metabolism using qRT-PCR analysis for RNA-seq validation. Specifically, four immune-related DEGs (STING, TBK1, WNT5, FABP), four anti-oxidative DEGs (SOD1, CAT, GSR, PRDX1, PRDX5), and three carbon metabolism-related DEGs (ENO4, PGD, GPI) were identified. These findings validate the reliability of the transcriptome database as a valuable resource for investigating host defense mechanisms against K. pneumoniae infection.

Furthermore, we examined the expression of specific immune and antioxidant-related genes, including SOD1, CAT, STING, TGF-β, IL-6, and IL-10, in various organ tissues using qRT-PCR (Fig. 5 B). The gene expression of SOD1, CAT, IL-6, and IL-10 in spleens and livers was significantly higher than in the control group (P < 0.05). However, there was no significant difference in gene expression in muscles, lung, and brain compared to the control group. Interestingly, the expression of STING and TGF-β was significantly higher in the liver, but there was no significant change in their expression in other organs.

| Original Text | Modified Text | Reason | |---|---|---| | In this study, to confirm the influence of the Mauremys mutica infection by Klebsiella pneumoniae. | In this study, we aimed to confirm the impact of Mauremys mutica infection caused by Klebsiella pneumoniae. | Improved clarity and concision by specifying the objective of the study. | | Four immune-related DEGs: STING, TBK1, WNT5, FABP. Four anti-oxidative DEGs: SOD1, CAT, GSR, PRDX1, PRDX5; and three carbon metabolism-related DEGs: ENO4, PGD, GPI, were identified using qRT-PCR analysis for RNA-seq validation. | We identified several differentially expressed genes (DEGs) related to immunity, antioxidant activity, and carbon metabolism using qRT-PCR analysis for RNA-seq validation. | Rewritten to improve readability and clarity. | | Subsequently, we obtained the expression of some immune and anti-oxidant related genes including SOD1, CAT, STING, TGF-β, IL-6, IL-10 by qRT-PCR at different organ tissues (Fig. 5 B). | Furthermore, we examined the expression of specific immune and antioxidant-related genes, including SOD1, CAT, STING, TGF-β, IL-6, and IL-10, in various organ tissues using qRT-PCR (Fig. 5 B). | Revised for improved clarity and concision. | | However, the expression of STING and TGF-β was significantly higher than control group in liver, but in other organs, There was no significant change in the expression of these genes. | However, the expression of STING and TGF-β was significantly higher in the liver, but there was no significant change in their expression in other organs. | Corrected grammar and sentence structure. |