In this study, an unidentified G-specific alginate lyase gene sequence was mined from the CAZy database, and the purified recombinant enzyme was obtained through heterologous expression for investigation of its enzymatic properties and kinetic constants. Additionally, UPSEC-DAD/MS and HPAEC-PAD/MS techniques were proposed to determine the degradation mode of the enzyme, including substrate cleavage sites, subsite specificity and cleavage modes, providing a clear and defined G-specific alginate lyase for research on alginate structure-activity relationships and targeted preparation of active oligosaccharides

我希望你能担任AI 写作导师起到英语翻译、拼写校对和修辞改进的角色。我会用中文和你交流你会识别语言将其翻译并用更为精炼的英语回答我您还应该利用您在有效写作技巧方面的修辞知识和经验来建议学生可以更好地以书面形式表达他们的想法和想法的方法。请仅回答更正和改进的部分不要写解释。请翻译我的第一句话是:本研究中一株未鉴定的G特异性褐藻胶裂解酶基因序列从CAZy数据库中被挖掘出来通过异源表达获得纯化重组酶并对

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